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mouse anti-human cd59 mem43/5  (Cedarlane)


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    Cedarlane mouse anti-human cd59 mem43/5
    Mouse Anti Human Cd59 Mem43/5, supplied by Cedarlane, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd59+mem43+5/cd59+antibody+labeled+fluorescein+isothiocyanate++fitc+/pm09737993-68-25-30
    Average 90 stars, based on 1 article reviews
    mouse anti-human cd59 mem43/5 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Western Blot:

    Article Title: Antibody Cross-linking of the Glycosylphosphatidylinositol-linked Protein CD59 on Hematopoietic Cells Induces Signaling Pathways Resembling Activation by Complement
    Article Snippet: All samples were analyzed by Western blotting after electrophoresis and transfer onto Immobilon-P polyvinylidene fluoride membranes (Millipore Corp., Mississauga, Canada). .. For CD59 Western blots, membranes were blocked for 1 h in 5% bovine serum albumin in PBS with 0.05% Tween 20 (PBSTw) and probed with mouse anti-human CD59 MEM43/5 (MONOSAN Cedarlane Laboratories Ltd., Hornby, Ontario, Canada) diluted 1:100 in blocking solution. .. Following several washes in PBSTw, blots were incubated in horseradish peroxidase-conjugated sheep anti-mouse IgG secondary antibody (Amersham Pharmacia Biotech, Oakville, Canada) diluted in blocking buffer for 30 min and were washed extensively in PBSTw before incubation in enhanced chemiluminescence detection reagents (ECL; Amersham Pharmacia Biotech) and exposure to x-ray film.

    Blocking Assay:

    Article Title: Antibody Cross-linking of the Glycosylphosphatidylinositol-linked Protein CD59 on Hematopoietic Cells Induces Signaling Pathways Resembling Activation by Complement
    Article Snippet: All samples were analyzed by Western blotting after electrophoresis and transfer onto Immobilon-P polyvinylidene fluoride membranes (Millipore Corp., Mississauga, Canada). .. For CD59 Western blots, membranes were blocked for 1 h in 5% bovine serum albumin in PBS with 0.05% Tween 20 (PBSTw) and probed with mouse anti-human CD59 MEM43/5 (MONOSAN Cedarlane Laboratories Ltd., Hornby, Ontario, Canada) diluted 1:100 in blocking solution. .. Following several washes in PBSTw, blots were incubated in horseradish peroxidase-conjugated sheep anti-mouse IgG secondary antibody (Amersham Pharmacia Biotech, Oakville, Canada) diluted in blocking buffer for 30 min and were washed extensively in PBSTw before incubation in enhanced chemiluminescence detection reagents (ECL; Amersham Pharmacia Biotech) and exposure to x-ray film.



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